General Cell Based Assays
Eurofins Discoverx PathHunter eXpress ErbB2/ErbB4 Dimerization Assay, 10pl Kit. (93-0960E3CP5L)
The PathHunter ErbB2/ErbB4 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. heregulin β-1) induced dimerization of ErbB2/ErbB4 receptors. This product uses EFC technology. The cell line included overexpresses ErbB2 target protein fused to PK alongside ErbB4 target protein fused to EA. Upon ligand-induced dimerization of ErbB2/ErbB4, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human CTACK (CCR10) β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human CTACK (CCR10) β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure CCR10 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human OPN5 (Opsin) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human OPN5 (Opsin-5) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure OPN5 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human AVPR1A Total GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human AVPR1A Stable Cell Line Total GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure AVPR1A (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Apexbio Technology LLC WST-8-Glucose-Uptake-Assay-Kit
WST-8 Glucose Uptake Assay Kit is used in the research category of metabolic assays originating from advancements in cell biology and biochemistry to facilitate the measurement of glucose uptake in cells The mechanism of action involves the reduction of WST-8 by dehydrogenases in cells resulting in a colorimetric change proportional to glucose uptake This assay commonly demonstrates in vitro activity in the nanomolar to low micromolar range enabling precise quantification of glucose consumption Typical applications include the study of insulin signaling pathways in various cell models evaluations in animal studies and drug screening for metabolic disorders Experimental concentrations typically depend on the specific research design allowing flexibility in adapting the assay for different experimental setups to understand cellular metabolism and assess potential therapeutic compounds
Eurofins Discoverx PathHunter CNR1 Bioassay Kit, 10-Plate. (93-0959Y2-00120)
The PathHunter CNR1 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. CP 55,940) based activation of CNR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CNR1 and EA-tagged β-Arrestin-2. Activation of CNR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress TGFBR1/ACVR2 Dimerization Assay, 2pl Kit. (93-1012E3CP5M)
The PathHunter TGFBR1/ACVR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF-11) induced dimerization of TGFBR1/ACVR2 receptors. This product uses EFC technology. The cell line included overexpresses TGFBR1 target protein fused to PK alongside ACVR2 target protein fused to EA. Upon ligand-induced dimerization of TGFBR1/ACVR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress RIPK2 Kinase Binding Assay, 2pl Kit. (96-0050E1CP0M)
The InCELL Hunter eXpress RIPK2 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of RIPK2 via detection of RIPK2 levels. This product uses EFC technology. The cell line included overexpresses RIPK2 target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cell Signaling Technology 7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit 1 Kit
7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit 1 Kit
Revvity Health Sciences Inc HTRF Human & Mouse Total VASP Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total VASP Detection Kit, 10,000 Assay Points
Eurofins Discoverx cAMP Hunter™ Human GABBR1 Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human GABBR1 Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GABBR1 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress UTR2 Total GPCR Internalization Assay, 10-Plate Kit. (93-0746E3CP5L)
The PathHunter UTR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of UTR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged UTR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the UTR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical NFAT AcTVatIon InHBTr III 50mg
A cell-permeant inhibitor of NFAT signaling; binds calcineurin (Kd = 0.8 µM), preventing it from dephosphorylating NFAT
Eurofins Discoverx InCELL Hunter eXpress Bcl2L1-Bax Protein Binding Assay, 10-plate Kit. (96-0009E3CP16L)
The InCELL Hunter eXpress Bcl2L1-Bax Protein Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that disrupt protein binding. This kit assesses compound-based disruption of Bcl2L1-Bax binding via detection of Bax levels. This product uses EFC technology. The cell line included overexpresses Bcl2L1 target in an untagged form and Bax binding partner fused to ProLabel. When a compound engages the Bcl2L1 target protein, partner binding is disrupted and the destabilized Bax is degraded, leading to a loss of EFC signal upon addition of EA containing detection reagent. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.